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These phases of mitosis are prophase, metaphase, anaphase, and telophase •i compared the two dyes to see which one was better at coloring the nucleus and allowing me to see the different phases of mitosis in the onion cells. In this chapter, you can use pictures of onion root tip cells to learn how to identify the different phases of mitosis and better understand what events occur during each phase of mitosis.
As mentioned, onion root tip cells divide rapidly as the roots elongate to absorb water and various minerals from the soil •in the lab, i cut the onion root tips and prepared them to be viewed under the microscope using two types of stains For this reason, it's possible to identify different stages of cell division by mitosis based on chromosomal distribution.
Microtubules align chromosomes along metaphase plate
Kinetochore microtubules shorten, pulling sister chromatids to opposite poles, polar microtubules elongate, lengthening dividing cell Nuclear membrane reforms, chromatin decondenses, and cell plate begins to form. During prophase, the chromosomes supercoil and the fibers of the spindle apparatus begin to form between centrosomes located at the pole of the cells The nuclear membrane also disintegrates at this time, freeing the chromosomes into the surrounding cytoplasm.
It is common to see photomicrographs of onion root cells when demonstrating how cell division takes place in plants Onions have larger chromosomes than most plants and stain dark The chromosomes are easily observed through a compound light microscope The cells pictured below are located in the apical meristem of the onion root.
These regions of growth are good for studying the cell cycle because at any given time, you can find cells that are undergoing mitosis
In order to examine cells in the tip of an onion root, a thin slice of the root is placed onto a microscope slide and stained so the chromosomes will be visible. Histology of mitosis in onion root tips (interphase, prophase, metaphase, anaphase, and telophase) stained with iron hematoxylin. Mastering prophase identification is essentially learning to read the earliest cues of cellular activity The answer lies in their unique biological characteristics.
These stages are prophase, metaphase, anaphase and telophase The reason why we are using an onion in this experiment is because their root tips grow at a rapid rate due to rapid cell division, which allows us to identify the different stages of mitosis.
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