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This test is intended to be used as an aid to the diagnosis of human granulocytic anaplasmosis (hga), a condition formerly known as human granulocytic ehrlichiosis (hge) caused by anaplasma phagocytophilum Specimens can be obtained from human serum or plasma. This test was developed and its performance characteristics determined by labcorp.
Pcr can be used to identify anaplasmosis from solid tissue and bone marrow specimens The mayo clinic pcr assay is capable of detecting and differentiating a phagocytophilum, e chaffeensis, e ewingii, and e muris eauclairensis. The reference standard serologic test for diagnosis of anaplasmosis is the indirect fluorescent antibody (ifa) test for immunoglobulin g (igg) using anaplasma phagocytophilum antigen.
Evaluating patients suspected of acute anaplasmosis or ehrlichiosis this test should not be used for screening asymptomatic individuals.
Specific primers and probe for anaplasma phagocytophilum are provided in the kit and can be detected through your real time thermal cycler by the 5’ nuclease pcr detection method During pcr amplification, forward and reverse primers hybridize to the anaplasma phagocytophilum target genomic dna. Freeze specimens promptly after collection Plasma must be separated from edta whole blood and frozen within 2 hours of collection
Ship refrigerated specimens to vrdl on cold packs Ship frozen specimens to vrdl on dry ice.
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