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This perspective discusses the concepts and approaches of de novo protein design, emerging challenges in designing structure and function, and the frontiers that lie ahead in deconstructing cellular processes with de novo proteins. The designed protein, based on the identified blueprint, was found to be a monomer with high thermal stability and exhibited atpase ability. Ai now allows us to design entirely new proteins de novo on a computer, predicting how they will fold and function
This powerful approach is yielding breakthroughs across biotechnology at an unprecedented pace. Here, we review recent innovations in major aspects of the de novo protein design and include how these advances were informed by principles of protein architecture and interactions derived from the wealth of structures in the protein data bank. For each pillar, we discuss the insights and strategies that have arisen to enable more accurate protein design and survey the key methods that have demonstrated our improved capacity to.
What protein folds are possible, and how do we access them?
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